Methods - Lipidomics-Regensburg

Fatty acids

Lipid class

Method

Stable isotope tracer

Lit.

Fatty acids – FA (double bond posit., cis/trans isomer separation)

GC-MS

¹³C-acetate or labeled FA

Ecker, J., M. Scherer, G. Schmitz, and G. Liebisch. 2012. J Chromatogr B Analyt Technol Biomed Life Sci. 897:98-104.

Ecker J, Liebisch G, Englmaier M, Grandl M, Robenek H, Schmitz G. Proc Natl Acad Sci U S A. 2010 Apr 27;107(17):7817-22.

Short chain fatty acids - SCFA

LC-MS/MS, Derivatization

Liebisch G, Ecker J, Roth S, Schweizer S, Öttl V, Schött HF, Yoon H, Haller D, Holler E, Burkhardt R, Matysik S.Biomolecules. 2019 Mar 28;9(4):121.

Glycerolipids

Lipid class	Method	Lit.
Diglycerides - DG	ESI+, FTMS	Höring M, Ejsing CS, Krautbauer S, Ertl VM, Burkhardt R, Liebisch G. J Lipid Res. 2021 Feb 16;62:100050.
Triglycerides - TG	ESI+, FTMS	Höring M, Ejsing CS, Krautbauer S, Ertl VM, Burkhardt R, Liebisch G. J Lipid Res. 2021 Feb 16;62:100050.

Glycerophospholipids

Lipid class	Method	Stable isotope tracer	Lit.
Bis(monoacylglycero)phosphate - BMP	LC, HILIC column, ESI+, MRM		Scherer, M., G. Schmitz, and G. Liebisch. 2010. Anal Chem. 82:8794-8799
Cardiolipin – CL
Phosphatidic acid - PA
Phosphatidylcholine - PC Sphingomyelin – SM	ESI+, PIS m/z 184 ESI+/-, FTMS	D₉-choline, PIS m/z 193 D₄-choline, PIS m/z 188	Liebisch, G., B. Lieser, J. Rathenberg, W. Drobnik, and G. Schmitz. 2004. Biochim Biophys Acta. 1686:108-117 Binder, M., G. Liebisch, T. Langmann, and G. Schmitz. 2006. J Biol Chem. 281:21869-21877. Höring M, Ekroos K, Baker PRS, Connell L, Stadler SC, Burkhardt R, Liebisch G. Anal Chem. 2020 Aug 18;92(16):10966-10970. Höring M, Ejsing CS, Krautbauer S, Ertl VM, Burkhardt R, Liebisch G. J Lipid Res. 2021 Feb 16;62:100050.
Phosphatidylethanolamine – PE	ESI+, NL m/z 141 ESI+/-, FTMS	D₄-ethanolamine, NL 145 ¹³C₂-ethanolamine, NL 143	Binder, M., G. Liebisch, T. Langmann, and G. Schmitz. 2006. J Biol Chem. 281:21869-21877. Höring M, Ekroos K, Baker PRS, Connell L, Stadler SC, Burkhardt R, Liebisch G. Anal Chem. 2020 Aug 18;92(16):10966-10970. Höring M, Ejsing CS, Krautbauer S, Ertl VM, Burkhardt R, Liebisch G. J Lipid Res. 2021 Feb 16;62:100050.
PE based Plasmalogens – PE P	ESI+ PIS m/z 364 (O-16:1) PIS m/z 392 (O-18:1) PIS m/z 390 (O-18:2)	D₄-ethanolamine, PIS m/z + 4	Zemski Berry KA, Murphy RC. J Am Soc Mass Spectrom. 2004 Oct;15(10):1499-508.
Phosphatidylinositol – PI	ESI+, NL 277		Matyash V, Liebisch G, Kurzchalia TV, Shevchenko A, Schwudke D. J Lipid Res. 2008 May; 49(5):1137-46.
Phosphatidylglycerol – PG	ESI+, NL 189 LC, HILIC column, ESI+, MRM		Matyash V, Liebisch G, Kurzchalia TV, Shevchenko A, Schwudke D. J Lipid Res. 2008 May; 49(5):1137-46 . Scherer, M., G. Schmitz, and G. Liebisch. 2010. Anal Chem. 82:8794-8799
Phosphatidylserine – PS	ESI+, NL 185	¹³C₃-serine, NL 188	Binder, M., G. Liebisch, T. Langmann, and G. Schmitz. 2006. J Biol Chem. 281:21869-21877.
Lysophosphatidylcholine – LPC	ESI+, PIS m/z 184	D₉-choline, PIS m/z 193	Liebisch, G., W. Drobnik, B. Lieser, and G. Schmitz. 2002. Clin Chem. 48:2217-2224. Höring M, Ejsing CS, Krautbauer S, Ertl VM, Burkhardt R, Liebisch G. J Lipid Res. 2021 Feb 16;62:100050.
Sphingosine-1-phosphate - S1P Lysophosphatidic acid - LPA	LC, HILIC column, ESI-, MRM		Scherer, M., G. Schmitz, and G. Liebisch. 2009. Clin Chem. 55:1218-1222.

Sphingolipids

Lipid class	Method	Lit.
Phosphatidylcholine - PC Sphingomyelin – SM	ESI+, PIS m/z 184 ESI+/-, FTMS	Liebisch, G., B. Lieser, J. Rathenberg, W. Drobnik, and G. Schmitz. 2004. Biochim Biophys Acta. 1686:108-117. Binder, M., G. Liebisch, T. Langmann, and G. Schmitz. 2006. J Biol Chem. 281:21869-21877. Höring M, Ejsing CS, Krautbauer S, Ertl VM, Burkhardt R, Liebisch G. J Lipid Res. 2021 Feb 16;62:100050.
Ceramides (d18:1) – Cer	ESI+, PIS m/z 264 ESI+/-, FTMS	Liebisch, G., W. Drobnik, M. Reil, B. Trumbach, R. Arnecke, B. Olgemoller, A. Roscher, and G. Schmitz. 1999. J Lipid Res. 40:1539-1546. Höring M, Ejsing CS, Krautbauer S, Ertl VM, Burkhardt R, Liebisch G. J Lipid Res. 2021 Feb 16;62:100050.
Ceramide-1-phosphate	LC, HILIC column, ESI+, MRM	Scherer, M., K. Leuthauser-Jaschinski, J. Ecker, G. Schmitz, and G. Liebisch. 2010. J Lipid Res. 51:2001-2011.
Sphingosylphosphorylcholine – SPC
Hexosyl-, Lactosylceramide
Sphingoid bases including methylated products – SPH
Sphingosine-1-phosphate - S1P Lysophosphatidic acid - LPA	LC, HILIC column, ESI-, MRM	Scherer, M., G. Schmitz, and G. Liebisch. 2009. Clin Chem. 55:1218-1222.

Sterols and steroids

Lipid class	Method	Lit.
Bile acids - BA	LC, RP column, ESI-, MRM	Scherer, M., C. Gnewuch, G. Schmitz, and G. Liebisch. 2009. J Chromatogr B Analyt Technol Biomed Life Sci. 877:3920-3925. Krautbauer S, Büchler C, Liebisch G. Anal Chem. 2016 Nov 15;88(22):10957-10961. Murine BA: Hermeling S, Plagge J, Krautbauer S, Ecker J, Burkhardt R, Liebisch G. Anal Bioanal Chem. 2025 Feb;417(4):687-696.
Free cholesterol - FC Cholesteryl ester – CE	ESI+, PIS m/z 369, Derivatization	Liebisch, G., M. Binder, R. Schifferer, T. Langmann, B. Schulz, G. Schmitz. 2006. Biochim Biophys Acta. 1761:121-128.
Free cholesterol - FC Cholesteryl ester – CE	ESI+, HRMS (MSX), FTMS	Höring M, Ejsing CS, Hermansson M, Liebisch G. Anal Chem. 2019 Mar 5;91(5):3459-3466. Höring M, Ejsing CS, Krautbauer S, Ertl VM, Burkhardt R, Liebisch G. J Lipid Res. 2021 Feb 16;62:100050.
Oxysterols	LC-MS/HighRes MS, ESI+, Derivatization	Pataj Z, Liebisch G, Schmitz G, Matysik S. J Chromatogr A. 2016 25;1439:82-8.
Oxysterols, plant sterols, and cholesterol precursors	GC-MS/MS	Matysik S, Klünemann HH, Schmitz G. Clin Chem. 2012 Nov;58(11):1557-64.
Steroid hormones	LC-MS/HighRes MS, ESI+,	Matysik S, Liebisch G. J Chromatogr A. 2017 Dec 1;1526:112-118.